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sterile cellophane membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad sterile cellophane membrane
    Sterile Cellophane Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sterile+cellophane+membrane/Cellophane+Membrane/pm41591149-163-21-24
    Average 99 stars, based on 115 article reviews
    sterile cellophane membrane - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sterility:

    Article Title: Influence of Major Environmental Parameters on Patulin Production by Penicillium expansum OM1 and Its Growth on Apple Puree Agar Media.
    Article Snippet: .. For fungal culture on APAM, 2 × 104 spores were inoculated onto each APAM agar plate, which was overlaid with a sterile cellophane membrane (Bio-rad, Hercules, CA, USA), after preparation of APAM agar plates including 40% apple puree by mixing homogenized apples (cultivar Fuji) with DW (homogenized apples: DW = 1:1.5 [w/w]) and adding agar into it. ..

    Article Title: Agrobacterium tumefaciens-mediated transformation for targeted disruption and over expression of genes in the poplar pathogenSphaerulina musiva
    Article Snippet: Sphaerulina musiva causes both leaf spots and cankers on poplar.. Leaf spots can lead to defoliation and cankers on branches and primary stems can lead to stem breakage and tree mortality.. The recent availability of both the S. musiva and Populus trichocarpa genomes offers a great opportunity to study host–pathogen interactions.

    Article Title: Botrytis euroamericana, a new species from peony and grape in North America and Europe.
    Article Snippet: A novel species of Botrytis isolated from peony in Alaska, USA and grape in Trento District, Italy was identified based on morphology, pathogenicity, and sequence data.. The grape and peony isolates share sequence homology in the glyceraldehyde-3-phosate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2), and necrosis and ethylene-inducing proteins 1 and 2 (NEP1 and NEP2) genes that place them in a distinct group closely related to B. aclada, a globally distributed pathogen of onions.. Genetic results were corroborated with morphological and pathogenicity trials that included two isolates of B. cinerea and two isolates B. paeoniae from peony in Alaska, and one isolate of B. aclada.

    Article Title: Plate assay for fungal enzymes using cellophane membranes.
    Article Snippet: 0003-2697/$ see front matter 2009 Elsevier Inc. A doi:10.1016/j.ab.2009.09.016 * Corresponding authors.. Fax: +886 5 632 8863 (C.Peng).. E-mail addresses: ctlo@nfu.edu.tw (C.-T.

    Article Title: Comparative quantitative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain mutant phenotypes and highlight interaction with cAMP and Ca 2+ signalling pathways.
    Article Snippet: .. Pre-cultures were made from calibrated explants (5 mm) deposited on plate with solid Sisler medium previously covered with a sterile cellophane membrane (Bio-Rad, USA). ..

    Article Title: Influence of Major Environmental Parameters on Patulin Production by Penicillium expansum OM1 and Its Growth on Apple Puree Agar Media
    Article Snippet: .. For fungal culture on APAM, 2 × 10 4 spores were inoculated onto each APAM agar plate, which was overlaid with a sterile cellophane membrane (Bio-rad, Hercules, CA, USA), after preparation of APAM agar plates including 40% apple puree by mixing homogenized apples (cultivar Fuji) with DW (homogenized apples: DW = 1:1.5 [ w / w ]) and adding agar into it. ..

    Article Title: Comparative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain loss of pathogenicity phenotypes and highlight interaction with cAMP and Ca 2+ signalling pathways
    Article Snippet: .. Pre-cultures were made from calibrated explants (5 mm) deposited on plate with solid Sisler medium previously covered with a sterile cellophane membrane (Bio-Rad, USA). ..

    Membrane:

    Article Title: Influence of Major Environmental Parameters on Patulin Production by Penicillium expansum OM1 and Its Growth on Apple Puree Agar Media.
    Article Snippet: .. For fungal culture on APAM, 2 × 104 spores were inoculated onto each APAM agar plate, which was overlaid with a sterile cellophane membrane (Bio-rad, Hercules, CA, USA), after preparation of APAM agar plates including 40% apple puree by mixing homogenized apples (cultivar Fuji) with DW (homogenized apples: DW = 1:1.5 [w/w]) and adding agar into it. ..

    Article Title: Agrobacterium tumefaciens-mediated transformation for targeted disruption and over expression of genes in the poplar pathogenSphaerulina musiva
    Article Snippet: Sphaerulina musiva causes both leaf spots and cankers on poplar.. Leaf spots can lead to defoliation and cankers on branches and primary stems can lead to stem breakage and tree mortality.. The recent availability of both the S. musiva and Populus trichocarpa genomes offers a great opportunity to study host–pathogen interactions.

    Article Title: Botrytis euroamericana, a new species from peony and grape in North America and Europe.
    Article Snippet: A novel species of Botrytis isolated from peony in Alaska, USA and grape in Trento District, Italy was identified based on morphology, pathogenicity, and sequence data.. The grape and peony isolates share sequence homology in the glyceraldehyde-3-phosate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2), and necrosis and ethylene-inducing proteins 1 and 2 (NEP1 and NEP2) genes that place them in a distinct group closely related to B. aclada, a globally distributed pathogen of onions.. Genetic results were corroborated with morphological and pathogenicity trials that included two isolates of B. cinerea and two isolates B. paeoniae from peony in Alaska, and one isolate of B. aclada.

    Article Title: Plate assay for fungal enzymes using cellophane membranes.
    Article Snippet: 0003-2697/$ see front matter 2009 Elsevier Inc. A doi:10.1016/j.ab.2009.09.016 * Corresponding authors.. Fax: +886 5 632 8863 (C.Peng).. E-mail addresses: ctlo@nfu.edu.tw (C.-T.

    Article Title: Comparative quantitative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain mutant phenotypes and highlight interaction with cAMP and Ca 2+ signalling pathways.
    Article Snippet: .. Pre-cultures were made from calibrated explants (5 mm) deposited on plate with solid Sisler medium previously covered with a sterile cellophane membrane (Bio-Rad, USA). ..

    Article Title: Influence of Major Environmental Parameters on Patulin Production by Penicillium expansum OM1 and Its Growth on Apple Puree Agar Media
    Article Snippet: .. For fungal culture on APAM, 2 × 10 4 spores were inoculated onto each APAM agar plate, which was overlaid with a sterile cellophane membrane (Bio-rad, Hercules, CA, USA), after preparation of APAM agar plates including 40% apple puree by mixing homogenized apples (cultivar Fuji) with DW (homogenized apples: DW = 1:1.5 [ w / w ]) and adding agar into it. ..

    Article Title: Comparative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain loss of pathogenicity phenotypes and highlight interaction with cAMP and Ca 2+ signalling pathways
    Article Snippet: .. Pre-cultures were made from calibrated explants (5 mm) deposited on plate with solid Sisler medium previously covered with a sterile cellophane membrane (Bio-Rad, USA). ..

    Cell Culture:

    Article Title: Agrobacterium tumefaciens-mediated transformation for targeted disruption and over expression of genes in the poplar pathogenSphaerulina musiva
    Article Snippet: Sphaerulina musiva causes both leaf spots and cankers on poplar.. Leaf spots can lead to defoliation and cankers on branches and primary stems can lead to stem breakage and tree mortality.. The recent availability of both the S. musiva and Populus trichocarpa genomes offers a great opportunity to study host–pathogen interactions.

    DNA Extraction:

    Article Title: Botrytis euroamericana, a new species from peony and grape in North America and Europe.
    Article Snippet: A novel species of Botrytis isolated from peony in Alaska, USA and grape in Trento District, Italy was identified based on morphology, pathogenicity, and sequence data.. The grape and peony isolates share sequence homology in the glyceraldehyde-3-phosate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2), and necrosis and ethylene-inducing proteins 1 and 2 (NEP1 and NEP2) genes that place them in a distinct group closely related to B. aclada, a globally distributed pathogen of onions.. Genetic results were corroborated with morphological and pathogenicity trials that included two isolates of B. cinerea and two isolates B. paeoniae from peony in Alaska, and one isolate of B. aclada.

    Polymerase Chain Reaction:

    Article Title: Botrytis euroamericana, a new species from peony and grape in North America and Europe.
    Article Snippet: A novel species of Botrytis isolated from peony in Alaska, USA and grape in Trento District, Italy was identified based on morphology, pathogenicity, and sequence data.. The grape and peony isolates share sequence homology in the glyceraldehyde-3-phosate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60), DNA-dependent RNA polymerase subunit II (RPB2), and necrosis and ethylene-inducing proteins 1 and 2 (NEP1 and NEP2) genes that place them in a distinct group closely related to B. aclada, a globally distributed pathogen of onions.. Genetic results were corroborated with morphological and pathogenicity trials that included two isolates of B. cinerea and two isolates B. paeoniae from peony in Alaska, and one isolate of B. aclada.



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    Image Search Results


    VdRid and VdDnmt5 positively regulate V. dahliae penetration activity partially through repression of Vd Rim15 expression. A Penetration ability assay. Colonies of V592, the knockout strains of Vd Rid , Vd Dnmt5 , and Vd Rim15 , as well as Vd Rim15oe strain grown on MM medium overlaid with a cellophane layer (above) and removal of the cellophane membrane (below). Photographs were taken at 3 and 8 dpi, respectively. B Detection of ROS production (upper panel) and intracellular Ca 2+ elevation in different strains. Different strains were grown on MM medium overlaid with the cellophane membrane for 2 days, and ROS production and intracellular Ca 2+ were detected with DCFH-DA and Fluo-4 AM, respectively. Fluorescence intensity represents the level of ROS and Ca 2+ . Bar = 20 μm. C Expression analysis of the VdCrz1 signaling-related genes in V592, Vd Δrid , Vd Δdnmt5 , Vd Δrid rim15 , Vd Δdnmt5 rim15 , Vd Δrim15 , and Vd Rim15 oe strains by RT-qPCR. Total RNA was isolated from hyphae grown on the cellophane membrane for 2 days. Data are the mean ± SD from three biological replicates. D Growth phenotype (upper panel) of different strains under H 2 O 2 (10 mM), high pH (10) and NaCl (0.5 M) treatments. Photographs were taken at 14 days post treatments. Column diagrams (lower panel) showing the measurement of colony area of different strains under the above stress treatments. For each strain, 3 samples were subjected to measurement of colony area. Each black dot represents a measure event. Gray horizontal lines represent the mean, and the error bars indicate ± SD from the number of measured events ( n = 3). One-way ANOVA test was performed. *** P < 0.001. **** P < 0.0001

    Journal: aBIOTECH

    Article Title: DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plants

    doi: 10.1007/s42994-023-00117-5

    Figure Lengend Snippet: VdRid and VdDnmt5 positively regulate V. dahliae penetration activity partially through repression of Vd Rim15 expression. A Penetration ability assay. Colonies of V592, the knockout strains of Vd Rid , Vd Dnmt5 , and Vd Rim15 , as well as Vd Rim15oe strain grown on MM medium overlaid with a cellophane layer (above) and removal of the cellophane membrane (below). Photographs were taken at 3 and 8 dpi, respectively. B Detection of ROS production (upper panel) and intracellular Ca 2+ elevation in different strains. Different strains were grown on MM medium overlaid with the cellophane membrane for 2 days, and ROS production and intracellular Ca 2+ were detected with DCFH-DA and Fluo-4 AM, respectively. Fluorescence intensity represents the level of ROS and Ca 2+ . Bar = 20 μm. C Expression analysis of the VdCrz1 signaling-related genes in V592, Vd Δrid , Vd Δdnmt5 , Vd Δrid rim15 , Vd Δdnmt5 rim15 , Vd Δrim15 , and Vd Rim15 oe strains by RT-qPCR. Total RNA was isolated from hyphae grown on the cellophane membrane for 2 days. Data are the mean ± SD from three biological replicates. D Growth phenotype (upper panel) of different strains under H 2 O 2 (10 mM), high pH (10) and NaCl (0.5 M) treatments. Photographs were taken at 14 days post treatments. Column diagrams (lower panel) showing the measurement of colony area of different strains under the above stress treatments. For each strain, 3 samples were subjected to measurement of colony area. Each black dot represents a measure event. Gray horizontal lines represent the mean, and the error bars indicate ± SD from the number of measured events ( n = 3). One-way ANOVA test was performed. *** P < 0.001. **** P < 0.0001

    Article Snippet: Sterilized cellophane membrane (Solarbio, YA0620) was overlaid onto MM medium.

    Techniques: Activity Assay, Expressing, Knock-Out, Membrane, Fluorescence, Quantitative RT-PCR, Isolation